Psicheck vector
WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … WebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价 格： ¥0. 货 号： 3544763. 产 地： 北京. 点击询问 我要采购. 竭诚为您服务！.
Psicheck vector
Did you know?
WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECK TM-2 vector, we found that seven of the eight sites in the Col4a3 – Col4a6 gene complementary to miR-29 were … WebpsiCHECK-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …
WebThe psiCheck-2 vector contains two luciferase genes, renilla as the experimental reporter while firefly the control reporter. Renilla is the experimental reporter as you can clone in regulatory ... WebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table …
WebApr 10, 2024 · PFN2 DNA sequences including or lacking predicted miRNA-binding sequence were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Transfection of the purified plasmid vectors into HNSCC cells were performed using Lipofectamine 2000 (Invitrogen) at 50 ng/well. After 48 h of transfection, we conducted dual luciferase … WebDual-luciferase reporter assay The psiCHECK-2 vector ( Promega, Cat # C8021) was used to construct plasmids for dual-luciferase reporter assay. RNF146 3′UTR or its mutant was cloned October 12, 2024 Zhaowei Wang, Xiaoling Xia, Jiaqi Li, Tatsushi Igaki
WebVector backbone psiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Vector type Luciferase Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name 3 miR-122 target sites Insert Size …
WebPsicheck Tm 2 Dual Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … fabletics wide leg sweatpantsWebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … fabletics yoga mat reviewWebDec 23, 2016 · (A) Schematic representation of dual luciferase reporter plasmid psiCHECK-2 vector containing the 3′ UTR of Dlx5 inserted downstream of Renilla luciferase gene. Four types of dual luciferase reporter plasmids were constructed, one wild type and three types of mutations were constructed within the seed region of the miR-203 and miR-320b ... fabletic tulip hem tankWebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … does initial entry training count for gi billWebDec 10, 2012 · To quantitatively determine the extent of repression, the expression of Renilla luciferase ( Rluc) from the psiCHECK-2 vector (psiCHECK) was used as a spliced mRNA model, through the introduction of a chimeric intron that was originally inserted to augment expression ( Fig. 1A ). fabletics x thredup bagWebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … does inisherin existWebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA … fabletics yitty uk