Phis2载体
http://www.youbio.cn/product/vt1637 Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a …
Phis2载体
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Webb12 apr. 2024 · 膜上载体蛋白属内在蛋白,它有选择地和膜一侧的分子或离子结合,形成载体-物质复合物,通过构象变化透过膜,把分子或离子释放到另一侧。 载体蛋白三种类型 . 1、单向运输载体:催化分子或离子单方向跨膜运输,顺电化学势差进行。 WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: …
Webb根据pHIS2载体上 Eco R Ⅰ和 Sac Ⅰ限制性内切酶识别位点的前后序列设计载体引物,进行PCR菌液检测,以阳性菌液为模板的PCR扩增的DNA条带要比以pHIS2空载体为模板的PCR扩增的条带分子量大20个碱基左右。 从 图 2 中可以看出,泳道1-13、15-18、19、21、23-26、28-30、32-37、40、41和43-48号的PCR扩增条带比对照分子量大,初步证明这些 … WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1).
Webb12 apr. 2024 · 2.启动子载体:pHIS2 3.prey载体:pGADT7 4.CLONTECH酵母单杂交系统 5.培养基: 1). 酵母完全培养基: YPDA:1% Yeast extract,2% Tryptone,2% … http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627
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WebbpHis2载体价格1000元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 dataframe apply expandhttp://www.zoonbio.com/molecular/yeast-one-hybrid.html bit of a brayWebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: … bit of a boreWebb8 juli 2024 · 载体的特点在于基因插入载体的构建简单,以及能够使用原养型尿嘧啶进行转化株 的筛选。 该载体包含以下元素: 1. 酵母 GAL1 启动子,能够在酿酒酵母中被半乳 … dataframe apply function to each cellWebb一、转化诱饵载体(参考Super转化试剂盒SK2402质粒转化步骤) 1.YPDA平板培养划线,活化Y187酵母菌种;挑酵母菌落于液体YPDA培养基摇菌培养; 2.制备感受态并 … dataframe append row deprecatedhttp://zomanbio.com/shengwu_info.php?nid=2549 bit of a broken clay potWebbpHis2酵母单杂交载体是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂,产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的pHis2酵 … dataframe apply vs applymap